WEB Immobilized enzymes for the sustainable degradation of plasticsTuesday (22.09.2020) 15:15 - 15:30 B: Biomaterials Part of:
Plastic is a unique material with many advantages like its low price, high versatility, light weight as well as high resistance. Therefore 350 to 400 million metric tons of plastics are produced every year, but their poor recyclability leads to an accumulation in terrestrial and marine environments. The negative consequences of plastic pollution for human and animal health and various ecosystems are enormous. The removal of plastics is a main challenge due to their high stability and durability. Therefore, enzymes have come into the focus of recent research because of their high selectivity and efficiency under mild conditions. Since, however, enzymes represent an expensive catalyst, a cost-efficient platform, whereby enzymes are attached on the surface of magnetic particles which can be magnetically extracted after their use and be re-used in several experiments would be advantageous. Therefore, ellipsoid shaped α-Fe2O3 where coated with SiO2 in a two-step sol-gel process. Afterwards the iron oxide core was reduced to magnetic Fe3O4 in a H2/argon atmosphere. Subsequently, the particles where modified with free amino groups that were used for the covalent attachment of enzymes, which was confirmed by zeta potential, IR and CHNS measurements. The activity of the enzyme was proven by optical conversion of 4-nitrophenyl acetate to 4-nitrophenol. Additionally, the enzymatic degradation of polycaprolactone fiber mats were analyzed regarding morphology changes.