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Keynote Lecture

WEB Carbon Nano Onions for Biomedical Applications

Tuesday (22.09.2020)
10:10 - 10:40 Z: Special Symposia I
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Multi-shell fullerenes, known as carbon nano-onions (CNOs), are structured by concentric shells of carbon atoms and are emerging as platforms for biomedical applications because of their ability to be internalized by cells and low toxicity. [1]


In my research group we have developed a synthetic multi-functionalisation strategy for the introduction of different functionalities (receptor targeting unit and imaging unit) onto the surface of the CNOs. The modified CNOs display high brightness and photostability in aqueous solutions and are selectively taken up by different cancer cell lines without significant cytotoxicity. [2]


We have also developed supramolecular functionalization with biocompatible polymers as an effective strategy to develop engineered drug carriers for targeted delivery applications. We reported the use of a hyaluronic acid-phospholipid (HA-DMPE) conjugate to target CD44 overexpressing cancer cells, while enhancing solubility of the nanoconstruct. Non-covalently functionalized CNOs with HA-DMPE show excellent in vitro cell viability in human breast carcinoma cells overexpressing CD44 and are uptaken to a greater extent compared to human ovarian carcinoma cells with an undetectable amount of CD44. In addition, they possess high in vivo biocompatibility in zebrafish during the different stages of development suggesting a high degree of biosafety of this class of nanomaterials. [3]


To probe the possible applications of CNOs as a platform for therapeutic and diagnostic interventions on CNS diseases, we injected fluorescent CNOs in vivo in mice hippocampus. We analyzed ex vivo their diffusion within brain tissues and their cellular localization by confocal and electron microscopy. The subsequent fluorescent staining of hippocampal cells populations indicates they efficiently internalize the nanoparticles. Furthermore, the inflammatory potential of the CNOs injection was found comparable to sterile vehicle infusion, and it did not result in manifest neurophysiological and behavioral alterations of hippocampal-mediated functions [4].


Our results encourage further development as targeted diagnostics or therapeutics nanocarriers.



References:

[1] S. Giordani et al., Current Medicinal Chemistry 2019, 26 (38), 6915.

[2] M. Frasconi et al., Chem Eur J 2015, 21 (52), 19071.

[3] M d’Amora et al., Colloids and Surfaces B: Biointerfaces, 2020, 110779.

[4] M. Trusel et al., ACS Appl. Mat. & Inter. 2018, 10 (20), 16952.

 

Speaker:
Prof. Silvia Giordani
Dublin City University