WEB In vitro assessment of boron-doped silicate and silicon oxycarbide bioactive glasses for bone tissue engineering
Bioactive glasses are inorganic materials being increasingly investigated for applications in bone regeneration due to their high surface reactivity leading to favorable interactions with body fluids during their dissolution process. The incorporation of therapeutic ions in the glass composition aims at enhancing the osteogenic and angiogenic performance of the material. Numerous biologically active ions are the focus of investigations1 with boron being considered in numerous studies as it promotes bone formation and stimulates angiogenesis. The effect of these ions in silicate glass systems has been the focus of extensive research. Bioactive glasses based on silicon oxycarbide and produced from a polymeric single-source precursor are a new family of inorganic materials with potential biomedical applications2. These alkali-free glasses are of interest due to their outstanding devitrification resistance and ability to retain their amorphous structure at high temperatures3. In this presentation, a new family of B doped silicate and SiOC bioactive glasses will be presented and compared. Silicon oxycarbide-based bioactive glasses were produced via thermal conversion (pyrolysis) and doped with B. In vitro activity was assessed by immersion tests in simulated body fluid (SBF) and samples were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). Cell culture studies were carried out to assess osteogenic and angiogenic effects of the glasses with focus on the influence of ion release kinetics. Osteoblast precursor cells (MC3T3-E1) were considered in indirect cell culture experiments. Specifically, the expression and secretion of vascular endothelial growth factor (VEGF) from cultured cells was measured and quantified. Additionally, the potential antibacterial effect of the glasses was evaluated against gram-negative and gram-positive bacteria E. Coli and S. Aureus respectively.
1. Hoppe A., et al., A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics, Biomaterials 2011, 32(11) 2757-2774.
2. Arango-Ospina, Marcela, et al. Silicon oxycarbide based materials for biomedical applications."Applied Materials Today (2019): 100482.
3. Gonzalo-Juan, I., et al., Synthesis and In Vitro Activity Assessment of Novel Silicon Oxycarbide-Based Bioactive Glasses, Materials 2016, 9(12), 959.