WEB Microcarrier functionalization for efficient stem cells productionThursday (24.09.2020) 11:35 - 11:50 B: Biomaterials Part of:
Stem cells are promising tools for new therapies used in neurodegenerative diseases and conditions, diabetes, heart disease, and others, but the culture (“proliferation”) of such cells in quantities that are sufficient for patient treatment still is expensive. A possible approach to improve the efficiency in the production of stem cells is the amplification in 3D bioreactors. In this case, small and dispersible microcarriers are needed as support for the cell attachment and growth, showing a higher specific surface than traditional 2D culture media.
The surface properties of these microcarriers play a key role in the proliferation of stem cells, since the interaction surface-cell must be strong enough to allow the cell adhesion and growth, but not too strong to be able to detach them intact. Previous studies have shown that surface modification of microcarriers can improve cell proliferation. Here, we show the surface modification of commercial polymer microcarriers (spherical particles about 0.15 mm) in two steps: activation of microcarriers by plasma treatment to generate reactive groups on the surface (Figure 1a) followed by chemical reaction that involves the formation of alkylsilane self-assembled monolayers on the surface. First tests have shown promising results in terms of cell growth on these materials (Figure 1b).
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